Coding

Part:BBa_K3019000:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)


GLC1 gene, complete cds, yeast codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 276
    Illegal AgeI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for S. cerevisiae. Illegal cutting site removing.


Source

glcI gene is from Arthrobacter sp. YCWD3. The sequence of the part was obtained from NCBI database (https://www.ncbi.nlm.nih.gov/nuccore/D23668.1?report=fasta) and codon optimised for S. Cerevisiae

References